Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_4_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_4_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61250
Pairs without alignments under any condition 21911
Pairs that did not map uniquely 16839
Genomic sequence context not extractable (edges of chromosomes) 0
61.3%21.9%16.8%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 2157029
Methylated C's in CpG context 28381
Methylated C's in CHG context 1690
Methylated C's in CHH context 7022
Methylated C's in Unknown context 85
Unmethylated C's in CpG context 246166
Unmethylated C's in CHG context 369285
Unmethylated C's in CHH context 1504485
Unmethylated C's in Unknown context 5278
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 15247 original top strand
CTOT 15316 complementary to original top strand
CTOB 15277 complementary to original bottom strand
OB 15410 original bottom strand
OTCTOTCTOBOB02k4k6k8k10k12k14k16k
Number of Alignments

Deduplication

Alignments analysed 61250
Unique alignments 57089
Duplicates removed 4161
Duplicated alignments were found at 3910 different positions
93.2%6.79%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 1371032
Methylated C's in CpG context 17454
Methylated C's in CHG context 1054
Methylated C's in CHH context 4473
Unmethylated C's in CpG context 155258
Unmethylated C's in CHG context 232556
Unmethylated C's in CHH context 960237
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801000204060801001000200030004000500060007000
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

20406080100020406080100050010001500200025003000350040004500
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls