Bismark report for: /gscratch/srlab/sr320/data/cg-big/zr3644_3_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_3_R2.fastp.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 61161 Mapping efficiency: 61.2% Sequence pairs with no alignments under any condition: 21806 Sequence pairs did not map uniquely: 17033 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 15370 ((converted) top strand) GA/CT/CT: 15366 (complementary to (converted) top strand) GA/CT/GA: 15264 (complementary to (converted) bottom strand) CT/GA/GA: 15161 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2154532 Total methylated C's in CpG context: 28478 Total methylated C's in CHG context: 1768 Total methylated C's in CHH context: 7387 Total methylated C's in Unknown context: 62 Total unmethylated C's in CpG context: 250025 Total unmethylated C's in CHG context: 372477 Total unmethylated C's in CHH context: 1494397 Total unmethylated C's in Unknown context: 5422 C methylated in CpG context: 10.2% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.1% Bismark completed in 0d 0h 0m 50s