Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61161 |
| Pairs without alignments under any condition |
21806 |
| Pairs that did not map uniquely |
17033 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2154532 |
| Methylated C's in CpG context |
28478 |
| Methylated C's in CHG context |
1768 |
| Methylated C's in CHH context |
7387 |
| Methylated C's in Unknown context |
62 |
| Unmethylated C's in CpG context |
250025 |
| Unmethylated C's in CHG context |
372477 |
| Unmethylated C's in CHH context |
1494397 |
| Unmethylated C's in Unknown context |
5422 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15370 |
original top strand |
| CTOT |
15366 |
complementary to original top strand |
| CTOB |
15264 |
complementary to original bottom strand |
| OB |
15161 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61161 |
| Unique alignments |
57491 |
| Duplicates removed |
3670 |
| Duplicated alignments were found at 3431 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1386996 |
| Methylated C's in CpG context |
17858 |
| Methylated C's in CHG context |
1119 |
| Methylated C's in CHH context |
4720 |
| Unmethylated C's in CpG context |
160089 |
| Unmethylated C's in CHG context |
237922 |
| Unmethylated C's in CHH context |
965288 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot