Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
61033 |
Pairs without alignments under any condition |
22427 |
Pairs that did not map uniquely |
16540 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2176242 |
Methylated C's in CpG context |
28402 |
Methylated C's in CHG context |
1868 |
Methylated C's in CHH context |
7614 |
Methylated C's in Unknown context |
76 |
Unmethylated C's in CpG context |
251780 |
Unmethylated C's in CHG context |
374557 |
Unmethylated C's in CHH context |
1512021 |
Unmethylated C's in Unknown context |
5381 |
Percentage methylation (CpG context) |
10.1% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15101 |
original top strand |
CTOT |
15016 |
complementary to original top strand |
CTOB |
15459 |
complementary to original bottom strand |
OB |
15457 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
61033 |
Unique alignments |
56894 |
Duplicates removed |
4139 |
Duplicated alignments were found at 3852 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1418146 |
Methylated C's in CpG context |
18079 |
Methylated C's in CHG context |
1195 |
Methylated C's in CHH context |
4889 |
Unmethylated C's in CpG context |
163292 |
Unmethylated C's in CHG context |
242611 |
Unmethylated C's in CHH context |
988080 |
Percentage methylation (CpG context) |
10.0% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot