Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_24_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_24_R2.fastp.fq.gz

Data processed at 22:22:30 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62315
Pairs without alignments under any condition 21022
Pairs that did not map uniquely 16663
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2207527
Methylated C's in CpG context 29017
Methylated C's in CHG context 1829
Methylated C's in CHH context 7614
Methylated C's in Unknown context 66
Unmethylated C's in CpG context 258161
Unmethylated C's in CHG context 383928
Unmethylated C's in CHH context 1526978
Unmethylated C's in Unknown context 5334
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15540 original top strand
CTOT 15661 complementary to original top strand
CTOB 15398 complementary to original bottom strand
OB 15716 original bottom strand

Deduplication

Alignments analysed 62315
Unique alignments 58133
Duplicates removed 4182
Duplicated alignments were found at 3943 different positions

Cytosine Methylation after Extraction

Total C's analysed 1405268
Methylated C's in CpG context 18046
Methylated C's in CHG context 1163
Methylated C's in CHH context 4781
Unmethylated C's in CpG context 163678
Unmethylated C's in CHG context 242659
Unmethylated C's in CHH context 974941
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot