Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61316 |
| Pairs without alignments under any condition |
21132 |
| Pairs that did not map uniquely |
17552 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2147258 |
| Methylated C's in CpG context |
28217 |
| Methylated C's in CHG context |
1894 |
| Methylated C's in CHH context |
7705 |
| Methylated C's in Unknown context |
40 |
| Unmethylated C's in CpG context |
251795 |
| Unmethylated C's in CHG context |
374551 |
| Unmethylated C's in CHH context |
1483096 |
| Unmethylated C's in Unknown context |
5236 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15304 |
original top strand |
| CTOT |
15128 |
complementary to original top strand |
| CTOB |
15103 |
complementary to original bottom strand |
| OB |
15781 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61316 |
| Unique alignments |
57209 |
| Duplicates removed |
4107 |
| Duplicated alignments were found at 3847 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1354323 |
| Methylated C's in CpG context |
17488 |
| Methylated C's in CHG context |
1183 |
| Methylated C's in CHH context |
4909 |
| Unmethylated C's in CpG context |
157505 |
| Unmethylated C's in CHG context |
233818 |
| Unmethylated C's in CHH context |
939420 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot