Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61839 |
| Pairs without alignments under any condition |
21374 |
| Pairs that did not map uniquely |
16787 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2188868 |
| Methylated C's in CpG context |
28781 |
| Methylated C's in CHG context |
1882 |
| Methylated C's in CHH context |
7621 |
| Methylated C's in Unknown context |
56 |
| Unmethylated C's in CpG context |
255932 |
| Unmethylated C's in CHG context |
381185 |
| Unmethylated C's in CHH context |
1513467 |
| Unmethylated C's in Unknown context |
5344 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15348 |
original top strand |
| CTOT |
15517 |
complementary to original top strand |
| CTOB |
15496 |
complementary to original bottom strand |
| OB |
15477 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61838 |
| Unique alignments |
57790 |
| Duplicates removed |
4048 |
| Duplicated alignments were found at 3784 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1400184 |
| Methylated C's in CpG context |
17967 |
| Methylated C's in CHG context |
1195 |
| Methylated C's in CHH context |
4901 |
| Unmethylated C's in CpG context |
162565 |
| Unmethylated C's in CHG context |
241491 |
| Unmethylated C's in CHH context |
972065 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot