Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_21_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_21_R2.fastp.fq.gz

Data processed at 22:22:29 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61355
Pairs without alignments under any condition 22075
Pairs that did not map uniquely 16570
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2163758
Methylated C's in CpG context 28956
Methylated C's in CHG context 1733
Methylated C's in CHH context 7474
Methylated C's in Unknown context 76
Unmethylated C's in CpG context 250171
Unmethylated C's in CHG context 375364
Unmethylated C's in CHH context 1500060
Unmethylated C's in Unknown context 5000
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15229 original top strand
CTOT 15342 complementary to original top strand
CTOB 15476 complementary to original bottom strand
OB 15307 original bottom strand

Deduplication

Alignments analysed 61354
Unique alignments 57158
Duplicates removed 4196
Duplicated alignments were found at 3916 different positions

Cytosine Methylation after Extraction

Total C's analysed 1396963
Methylated C's in CpG context 18229
Methylated C's in CHG context 1108
Methylated C's in CHH context 4713
Unmethylated C's in CpG context 160395
Unmethylated C's in CHG context 240305
Unmethylated C's in CHH context 972213
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot