Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61594 |
| Pairs without alignments under any condition |
21962 |
| Pairs that did not map uniquely |
16444 |
| Genomic sequence context not extractable (edges of chromosomes) |
2 |
|
|
Cytosine Methylation
| Total C's analysed |
2172583 |
| Methylated C's in CpG context |
28191 |
| Methylated C's in CHG context |
1786 |
| Methylated C's in CHH context |
8067 |
| Methylated C's in Unknown context |
91 |
| Unmethylated C's in CpG context |
252733 |
| Unmethylated C's in CHG context |
376351 |
| Unmethylated C's in CHH context |
1505455 |
| Unmethylated C's in Unknown context |
5247 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15039 |
original top strand |
| CTOT |
15791 |
complementary to original top strand |
| CTOB |
15787 |
complementary to original bottom strand |
| OB |
14975 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61592 |
| Unique alignments |
57403 |
| Duplicates removed |
4189 |
| Duplicated alignments were found at 3896 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1402572 |
| Methylated C's in CpG context |
17549 |
| Methylated C's in CHG context |
1151 |
| Methylated C's in CHH context |
5152 |
| Unmethylated C's in CpG context |
162343 |
| Unmethylated C's in CHG context |
241099 |
| Unmethylated C's in CHH context |
975278 |
| Percentage methylation (CpG context) |
9.8% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot