Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
62534 |
| Pairs without alignments under any condition |
20244 |
| Pairs that did not map uniquely |
17222 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2197155 |
| Methylated C's in CpG context |
26938 |
| Methylated C's in CHG context |
1753 |
| Methylated C's in CHH context |
7196 |
| Methylated C's in Unknown context |
58 |
| Unmethylated C's in CpG context |
258523 |
| Unmethylated C's in CHG context |
384627 |
| Unmethylated C's in CHH context |
1518118 |
| Unmethylated C's in Unknown context |
5416 |
| Percentage methylation (CpG context) |
9.4% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15602 |
original top strand |
| CTOT |
15606 |
complementary to original top strand |
| CTOB |
15523 |
complementary to original bottom strand |
| OB |
15803 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
62534 |
| Unique alignments |
58412 |
| Duplicates removed |
4122 |
| Duplicated alignments were found at 3862 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1373705 |
| Methylated C's in CpG context |
16457 |
| Methylated C's in CHG context |
1093 |
| Methylated C's in CHH context |
4561 |
| Unmethylated C's in CpG context |
160479 |
| Unmethylated C's in CHG context |
238266 |
| Unmethylated C's in CHH context |
952849 |
| Percentage methylation (CpG context) |
9.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot