Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3644_16_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3644_16_R2.fastp.fq.gz

Data processed at 22:22:29 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61736
Pairs without alignments under any condition 20553
Pairs that did not map uniquely 17711
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2142951
Methylated C's in CpG context 29209
Methylated C's in CHG context 1885
Methylated C's in CHH context 7823
Methylated C's in Unknown context 69
Unmethylated C's in CpG context 250460
Unmethylated C's in CHG context 373429
Unmethylated C's in CHH context 1480145
Unmethylated C's in Unknown context 5147
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15421 original top strand
CTOT 15520 complementary to original top strand
CTOB 15692 complementary to original bottom strand
OB 15103 original bottom strand

Deduplication

Alignments analysed 61736
Unique alignments 57429
Duplicates removed 4307
Duplicated alignments were found at 4033 different positions

Cytosine Methylation after Extraction

Total C's analysed 1314284
Methylated C's in CpG context 17404
Methylated C's in CHG context 1122
Methylated C's in CHH context 4762
Unmethylated C's in CpG context 152417
Unmethylated C's in CHG context 227151
Unmethylated C's in CHH context 911428
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot