Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61418 |
| Pairs without alignments under any condition |
21905 |
| Pairs that did not map uniquely |
16677 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2166668 |
| Methylated C's in CpG context |
30900 |
| Methylated C's in CHG context |
1839 |
| Methylated C's in CHH context |
7454 |
| Methylated C's in Unknown context |
91 |
| Unmethylated C's in CpG context |
248157 |
| Unmethylated C's in CHG context |
374324 |
| Unmethylated C's in CHH context |
1503994 |
| Unmethylated C's in Unknown context |
5309 |
| Percentage methylation (CpG context) |
11.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15110 |
original top strand |
| CTOT |
15436 |
complementary to original top strand |
| CTOB |
15403 |
complementary to original bottom strand |
| OB |
15469 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61418 |
| Unique alignments |
57436 |
| Duplicates removed |
3982 |
| Duplicated alignments were found at 3767 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1385235 |
| Methylated C's in CpG context |
19228 |
| Methylated C's in CHG context |
1169 |
| Methylated C's in CHH context |
4747 |
| Unmethylated C's in CpG context |
157318 |
| Unmethylated C's in CHG context |
237701 |
| Unmethylated C's in CHH context |
965072 |
| Percentage methylation (CpG context) |
10.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot