Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61154 |
| Pairs without alignments under any condition |
21890 |
| Pairs that did not map uniquely |
16956 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2154179 |
| Methylated C's in CpG context |
29648 |
| Methylated C's in CHG context |
1760 |
| Methylated C's in CHH context |
7608 |
| Methylated C's in Unknown context |
68 |
| Unmethylated C's in CpG context |
251436 |
| Unmethylated C's in CHG context |
375780 |
| Unmethylated C's in CHH context |
1487947 |
| Unmethylated C's in Unknown context |
5343 |
| Percentage methylation (CpG context) |
10.5% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15210 |
original top strand |
| CTOT |
15214 |
complementary to original top strand |
| CTOB |
15355 |
complementary to original bottom strand |
| OB |
15374 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61153 |
| Unique alignments |
57147 |
| Duplicates removed |
4006 |
| Duplicated alignments were found at 3715 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1367044 |
| Methylated C's in CpG context |
18292 |
| Methylated C's in CHG context |
1099 |
| Methylated C's in CHH context |
4850 |
| Unmethylated C's in CpG context |
158774 |
| Unmethylated C's in CHG context |
236251 |
| Unmethylated C's in CHH context |
947778 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot