Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3534_9_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3534_9_R2.fastp.fq.gz

Data processed at 22:22:28 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61885
Pairs without alignments under any condition 21378
Pairs that did not map uniquely 16737
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2175764
Methylated C's in CpG context 28518
Methylated C's in CHG context 1819
Methylated C's in CHH context 7613
Methylated C's in Unknown context 87
Unmethylated C's in CpG context 256743
Unmethylated C's in CHG context 382839
Unmethylated C's in CHH context 1498232
Unmethylated C's in Unknown context 5430
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15407 original top strand
CTOT 15476 complementary to original top strand
CTOB 15624 complementary to original bottom strand
OB 15377 original bottom strand

Deduplication

Alignments analysed 61884
Unique alignments 55727
Duplicates removed 6157
Duplicated alignments were found at 5596 different positions

Cytosine Methylation after Extraction

Total C's analysed 1352824
Methylated C's in CpG context 17147
Methylated C's in CHG context 1109
Methylated C's in CHH context 4655
Unmethylated C's in CpG context 158036
Unmethylated C's in CHG context 235705
Unmethylated C's in CHH context 936172
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot