Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3534_7_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3534_7_R2.fastp.fq.gz

Data processed at 22:22:28 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61281
Pairs without alignments under any condition 23002
Pairs that did not map uniquely 15717
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2136176
Methylated C's in CpG context 28095
Methylated C's in CHG context 1704
Methylated C's in CHH context 7936
Methylated C's in Unknown context 89
Unmethylated C's in CpG context 244900
Unmethylated C's in CHG context 369082
Unmethylated C's in CHH context 1484459
Unmethylated C's in Unknown context 5394
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15063 original top strand
CTOT 15408 complementary to original top strand
CTOB 15464 complementary to original bottom strand
OB 15346 original bottom strand

Deduplication

Alignments analysed 61281
Unique alignments 55478
Duplicates removed 5803
Duplicated alignments were found at 5250 different positions

Cytosine Methylation after Extraction

Total C's analysed 1350779
Methylated C's in CpG context 17175
Methylated C's in CHG context 1062
Methylated C's in CHH context 5015
Unmethylated C's in CpG context 153801
Unmethylated C's in CHG context 231013
Unmethylated C's in CHH context 942713
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot