Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zr3534_4_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/zr3534_4_R2.fastp.fq.gz

Data processed at 22:22:27 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61752
Pairs without alignments under any condition 22130
Pairs that did not map uniquely 16118
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2153460
Methylated C's in CpG context 31225
Methylated C's in CHG context 1738
Methylated C's in CHH context 8146
Methylated C's in Unknown context 84
Unmethylated C's in CpG context 248966
Unmethylated C's in CHG context 375718
Unmethylated C's in CHH context 1487667
Unmethylated C's in Unknown context 5212
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15331 original top strand
CTOT 15379 complementary to original top strand
CTOB 15518 complementary to original bottom strand
OB 15523 original bottom strand

Deduplication

Alignments analysed 61751
Unique alignments 55259
Duplicates removed 6492
Duplicated alignments were found at 5909 different positions

Cytosine Methylation after Extraction

Total C's analysed 1334307
Methylated C's in CpG context 18547
Methylated C's in CHG context 1068
Methylated C's in CHH context 4983
Unmethylated C's in CpG context 153522
Unmethylated C's in CHG context 230168
Unmethylated C's in CHH context 926019
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot