Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
60805 |
| Pairs without alignments under any condition |
23295 |
| Pairs that did not map uniquely |
15900 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2125117 |
| Methylated C's in CpG context |
32587 |
| Methylated C's in CHG context |
1966 |
| Methylated C's in CHH context |
8237 |
| Methylated C's in Unknown context |
79 |
| Unmethylated C's in CpG context |
241674 |
| Unmethylated C's in CHG context |
368620 |
| Unmethylated C's in CHH context |
1472033 |
| Unmethylated C's in Unknown context |
5484 |
| Percentage methylation (CpG context) |
11.9% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15213 |
original top strand |
| CTOT |
15176 |
complementary to original top strand |
| CTOB |
15206 |
complementary to original bottom strand |
| OB |
15210 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
60805 |
| Unique alignments |
54955 |
| Duplicates removed |
5850 |
| Duplicated alignments were found at 5361 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1361137 |
| Methylated C's in CpG context |
20085 |
| Methylated C's in CHG context |
1230 |
| Methylated C's in CHH context |
5181 |
| Unmethylated C's in CpG context |
153239 |
| Unmethylated C's in CHG context |
233513 |
| Unmethylated C's in CHH context |
947889 |
| Percentage methylation (CpG context) |
11.6% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot