Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
60658 |
| Pairs without alignments under any condition |
29074 |
| Pairs that did not map uniquely |
10268 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2633532 |
| Methylated C's in CpG context |
38086 |
| Methylated C's in CHG context |
3652 |
| Methylated C's in CHH context |
16168 |
| Methylated C's in Unknown context |
288 |
| Unmethylated C's in CpG context |
276138 |
| Unmethylated C's in CHG context |
427634 |
| Unmethylated C's in CHH context |
1871854 |
| Unmethylated C's in Unknown context |
7589 |
| Percentage methylation (CpG context) |
12.1% |
| Percentage methylation (CHG context) |
0.8% |
| Percentage methylation (CHH context) |
0.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
30312 |
original top strand |
| CTOT |
6 |
complementary to original top strand |
| CTOB |
6 |
complementary to original bottom strand |
| OB |
30334 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
60658 |
| Unique alignments |
57479 |
| Duplicates removed |
3179 |
| Duplicated alignments were found at 3088 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1978606 |
| Methylated C's in CpG context |
28775 |
| Methylated C's in CHG context |
3057 |
| Methylated C's in CHH context |
13217 |
| Unmethylated C's in CpG context |
207515 |
| Unmethylated C's in CHG context |
321019 |
| Unmethylated C's in CHH context |
1405023 |
| Percentage methylation (CpG context) |
12.2% |
| Percentage methylation (CHG context) |
0.9% |
| Percentage methylation (CHH context) |
0.9% |
|
|
M-Bias Plot