Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/5901_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/5901_R2.fastp.fq.gz

Data processed at 22:22:26 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 59788
Pairs without alignments under any condition 29986
Pairs that did not map uniquely 10226
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2557483
Methylated C's in CpG context 41376
Methylated C's in CHG context 3779
Methylated C's in CHH context 16013
Methylated C's in Unknown context 242
Unmethylated C's in CpG context 269479
Unmethylated C's in CHG context 418335
Unmethylated C's in CHH context 1808501
Unmethylated C's in Unknown context 7599
Percentage methylation (CpG context) 13.3%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29983 original top strand
CTOT 8 complementary to original top strand
CTOB 10 complementary to original bottom strand
OB 29787 original bottom strand

Deduplication

Alignments analysed 59788
Unique alignments 56513
Duplicates removed 3275
Duplicated alignments were found at 3170 different positions

Cytosine Methylation after Extraction

Total C's analysed 1891864
Methylated C's in CpG context 30700
Methylated C's in CHG context 3083
Methylated C's in CHH context 13000
Unmethylated C's in CpG context 199778
Unmethylated C's in CHG context 309087
Unmethylated C's in CHH context 1336216
Percentage methylation (CpG context) 13.3%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.0%

M-Bias Plot