Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/5203_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/5203_R2.fastp.fq.gz

Data processed at 22:22:26 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 58371
Pairs without alignments under any condition 31612
Pairs that did not map uniquely 10017
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2443828
Methylated C's in CpG context 39076
Methylated C's in CHG context 3876
Methylated C's in CHH context 17506
Methylated C's in Unknown context 272
Unmethylated C's in CpG context 251416
Unmethylated C's in CHG context 392308
Unmethylated C's in CHH context 1739646
Unmethylated C's in Unknown context 7294
Percentage methylation (CpG context) 13.5%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29260 original top strand
CTOT 14 complementary to original top strand
CTOB 17 complementary to original bottom strand
OB 29080 original bottom strand

Deduplication

Alignments analysed 58371
Unique alignments 55643
Duplicates removed 2728
Duplicated alignments were found at 2653 different positions

Cytosine Methylation after Extraction

Total C's analysed 1813834
Methylated C's in CpG context 29295
Methylated C's in CHG context 3166
Methylated C's in CHH context 14301
Unmethylated C's in CpG context 186579
Unmethylated C's in CHG context 290961
Unmethylated C's in CHH context 1289532
Percentage methylation (CpG context) 13.6%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.1%

M-Bias Plot