Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/5201_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/5201_R2.fastp.fq.gz

Data processed at 22:22:26 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60895
Pairs without alignments under any condition 28803
Pairs that did not map uniquely 10302
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2482069
Methylated C's in CpG context 35234
Methylated C's in CHG context 2928
Methylated C's in CHH context 13361
Methylated C's in Unknown context 286
Unmethylated C's in CpG context 254332
Unmethylated C's in CHG context 390650
Unmethylated C's in CHH context 1785564
Unmethylated C's in Unknown context 7876
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.7%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 30438 original top strand
CTOT 11 complementary to original top strand
CTOB 7 complementary to original bottom strand
OB 30439 original bottom strand

Deduplication

Alignments analysed 60895
Unique alignments 57823
Duplicates removed 3072
Duplicated alignments were found at 2996 different positions

Cytosine Methylation after Extraction

Total C's analysed 1739031
Methylated C's in CpG context 25095
Methylated C's in CHG context 2450
Methylated C's in CHH context 10982
Unmethylated C's in CpG context 178413
Unmethylated C's in CHG context 273483
Unmethylated C's in CHH context 1248608
Percentage methylation (CpG context) 12.3%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%

M-Bias Plot