Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
57332 |
| Pairs without alignments under any condition |
32794 |
| Pairs that did not map uniquely |
9874 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2445297 |
| Methylated C's in CpG context |
38255 |
| Methylated C's in CHG context |
3452 |
| Methylated C's in CHH context |
15550 |
| Methylated C's in Unknown context |
243 |
| Unmethylated C's in CpG context |
252250 |
| Unmethylated C's in CHG context |
395300 |
| Unmethylated C's in CHH context |
1740490 |
| Unmethylated C's in Unknown context |
7083 |
| Percentage methylation (CpG context) |
13.2% |
| Percentage methylation (CHG context) |
0.9% |
| Percentage methylation (CHH context) |
0.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
28576 |
original top strand |
| CTOT |
6 |
complementary to original top strand |
| CTOB |
8 |
complementary to original bottom strand |
| OB |
28742 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
57332 |
| Unique alignments |
54898 |
| Duplicates removed |
2434 |
| Duplicated alignments were found at 2364 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1846095 |
| Methylated C's in CpG context |
29111 |
| Methylated C's in CHG context |
2883 |
| Methylated C's in CHH context |
12836 |
| Unmethylated C's in CpG context |
190000 |
| Unmethylated C's in CHG context |
297786 |
| Unmethylated C's in CHH context |
1313479 |
| Percentage methylation (CpG context) |
13.3% |
| Percentage methylation (CHG context) |
1.0% |
| Percentage methylation (CHH context) |
1.0% |
|
|
M-Bias Plot