Bismark report for: /gscratch/srlab/sr320/data/cg-big/3502_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/3502_R2.fastp.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 61008 Mapping efficiency: 61.0% Sequence pairs with no alignments under any condition: 28396 Sequence pairs did not map uniquely: 10596 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 30455 ((converted) top strand) GA/CT/CT: 5 (complementary to (converted) top strand) GA/CT/GA: 9 (complementary to (converted) bottom strand) CT/GA/GA: 30539 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2617130 Total methylated C's in CpG context: 38787 Total methylated C's in CHG context: 3814 Total methylated C's in CHH context: 16132 Total methylated C's in Unknown context: 318 Total unmethylated C's in CpG context: 272956 Total unmethylated C's in CHG context: 424987 Total unmethylated C's in CHH context: 1860454 Total unmethylated C's in Unknown context: 7826 C methylated in CpG context: 12.4% C methylated in CHG context: 0.9% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 3.9% Bismark completed in 0d 0h 0m 55s