Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/3502_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/3502_R2.fastp.fq.gz

Data processed at 22:22:26 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61008
Pairs without alignments under any condition 28396
Pairs that did not map uniquely 10596
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2617130
Methylated C's in CpG context 38787
Methylated C's in CHG context 3814
Methylated C's in CHH context 16132
Methylated C's in Unknown context 318
Unmethylated C's in CpG context 272956
Unmethylated C's in CHG context 424987
Unmethylated C's in CHH context 1860454
Unmethylated C's in Unknown context 7826
Percentage methylation (CpG context) 12.4%
Percentage methylation (CHG context) 0.9%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 30455 original top strand
CTOT 5 complementary to original top strand
CTOB 9 complementary to original bottom strand
OB 30539 original bottom strand

Deduplication

Alignments analysed 61008
Unique alignments 57719
Duplicates removed 3289
Duplicated alignments were found at 3173 different positions

Cytosine Methylation after Extraction

Total C's analysed 1914887
Methylated C's in CpG context 28773
Methylated C's in CHG context 3084
Methylated C's in CHH context 12967
Unmethylated C's in CpG context 199776
Unmethylated C's in CHG context 310413
Unmethylated C's in CHH context 1359874
Percentage methylation (CpG context) 12.6%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 0.9%

M-Bias Plot