Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
59416 |
| Pairs without alignments under any condition |
30587 |
| Pairs that did not map uniquely |
9997 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2464248 |
| Methylated C's in CpG context |
40925 |
| Methylated C's in CHG context |
3621 |
| Methylated C's in CHH context |
17614 |
| Methylated C's in Unknown context |
284 |
| Unmethylated C's in CpG context |
249148 |
| Unmethylated C's in CHG context |
395141 |
| Unmethylated C's in CHH context |
1757799 |
| Unmethylated C's in Unknown context |
7377 |
| Percentage methylation (CpG context) |
14.1% |
| Percentage methylation (CHG context) |
0.9% |
| Percentage methylation (CHH context) |
1.0% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
29711 |
original top strand |
| CTOT |
9 |
complementary to original top strand |
| CTOB |
20 |
complementary to original bottom strand |
| OB |
29676 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
59416 |
| Unique alignments |
56422 |
| Duplicates removed |
2994 |
| Duplicated alignments were found at 2901 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1849861 |
| Methylated C's in CpG context |
31012 |
| Methylated C's in CHG context |
3012 |
| Methylated C's in CHH context |
14206 |
| Unmethylated C's in CpG context |
187357 |
| Unmethylated C's in CHG context |
296163 |
| Unmethylated C's in CHH context |
1318111 |
| Percentage methylation (CpG context) |
14.2% |
| Percentage methylation (CHG context) |
1.0% |
| Percentage methylation (CHH context) |
1.1% |
|
|
M-Bias Plot