Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
59284 |
| Pairs without alignments under any condition |
30449 |
| Pairs that did not map uniquely |
10267 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2538444 |
| Methylated C's in CpG context |
39048 |
| Methylated C's in CHG context |
4019 |
| Methylated C's in CHH context |
17093 |
| Methylated C's in Unknown context |
324 |
| Unmethylated C's in CpG context |
261599 |
| Unmethylated C's in CHG context |
413167 |
| Unmethylated C's in CHH context |
1803518 |
| Unmethylated C's in Unknown context |
7724 |
| Percentage methylation (CpG context) |
13.0% |
| Percentage methylation (CHG context) |
1.0% |
| Percentage methylation (CHH context) |
0.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
29531 |
original top strand |
| CTOT |
8 |
complementary to original top strand |
| CTOB |
2 |
complementary to original bottom strand |
| OB |
29743 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
59284 |
| Unique alignments |
56460 |
| Duplicates removed |
2824 |
| Duplicated alignments were found at 2747 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1880911 |
| Methylated C's in CpG context |
29142 |
| Methylated C's in CHG context |
3310 |
| Methylated C's in CHH context |
13974 |
| Unmethylated C's in CpG context |
193905 |
| Unmethylated C's in CHG context |
305640 |
| Unmethylated C's in CHH context |
1334940 |
| Percentage methylation (CpG context) |
13.1% |
| Percentage methylation (CHG context) |
1.1% |
| Percentage methylation (CHH context) |
1.0% |
|
|
M-Bias Plot