Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/0502_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/0502_R2.fastp.fq.gz

Data processed at 22:22:25 on 2021-06-08


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 59284
Pairs without alignments under any condition 30449
Pairs that did not map uniquely 10267
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2538444
Methylated C's in CpG context 39048
Methylated C's in CHG context 4019
Methylated C's in CHH context 17093
Methylated C's in Unknown context 324
Unmethylated C's in CpG context 261599
Unmethylated C's in CHG context 413167
Unmethylated C's in CHH context 1803518
Unmethylated C's in Unknown context 7724
Percentage methylation (CpG context) 13.0%
Percentage methylation (CHG context) 1.0%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 29531 original top strand
CTOT 8 complementary to original top strand
CTOB 2 complementary to original bottom strand
OB 29743 original bottom strand

Deduplication

Alignments analysed 59284
Unique alignments 56460
Duplicates removed 2824
Duplicated alignments were found at 2747 different positions

Cytosine Methylation after Extraction

Total C's analysed 1880911
Methylated C's in CpG context 29142
Methylated C's in CHG context 3310
Methylated C's in CHH context 13974
Unmethylated C's in CpG context 193905
Unmethylated C's in CHG context 305640
Unmethylated C's in CHH context 1334940
Percentage methylation (CpG context) 13.1%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.0%

M-Bias Plot