Bismark report for: /gscratch/srlab/sr320/data/cg-big/0502_R1.fastp.fq.gz and /gscratch/srlab/sr320/data/cg-big/0502_R2.fastp.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 58618241 Number of paired-end alignments with a unique best hit: 34683710 Mapping efficiency: 59.2% Sequence pairs with no alignments under any condition: 18011074 Sequence pairs did not map uniquely: 5923457 Sequence pairs which were discarded because genomic sequence could not be extracted: 122 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 17340208 ((converted) top strand) GA/CT/CT: 3654 (complementary to (converted) top strand) GA/CT/GA: 4778 (complementary to (converted) bottom strand) CT/GA/GA: 17334948 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1484454964 Total methylated C's in CpG context: 22326558 Total methylated C's in CHG context: 2339845 Total methylated C's in CHH context: 10359171 Total methylated C's in Unknown context: 188436 Total unmethylated C's in CpG context: 154228298 Total unmethylated C's in CHG context: 241093267 Total unmethylated C's in CHH context: 1054107825 Total unmethylated C's in Unknown context: 4382777 C methylated in CpG context: 12.6% C methylated in CHG context: 1.0% C methylated in CHH context: 1.0% C methylated in unknown context (CN or CHN): 4.1% Bismark completed in 0d 6h 19m 53s