Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/8_ACTTGA_L001_R1_001_trimmed.fq.gz

Data processed at 16:19:35 on 2021-06-03


Alignment Stats

Sequences analysed in total 13949213
Single-end alignments with a unique best hit 6487669
Sequences without alignments under any condition 214634
Sequences that did not map uniquely 7246910
Genomic sequence context not extractable (edges of chromosomes) 4632

Cytosine Methylation

Total C's analysed 36776252
Methylated C's in CpG context 1160722
Methylated C's in CHG context 164820
Methylated C's in CHH context 3984847
Methylated C's in Unknown context 69576
Unmethylated C's in CpG context 3227776
Unmethylated C's in CHG context 5765497
Unmethylated C's in CHH context 22472590
Unmethylated C's in Unknown context 122119
Percentage methylation (CpG context) 26.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 15.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2531677 original top strand
CTOT 712380 complementary to original top strand
CTOB 713725 complementary to original bottom strand
OB 2525255 original bottom strand

Deduplication

Alignments analysed 6483037
Unique alignments 5588889
Duplicates removed 894148
Duplicated alignments were found at 536263 different positions

Cytosine Methylation after Extraction

Total C's analysed 31571331
Methylated C's in CpG context 981149
Methylated C's in CHG context 137864
Methylated C's in CHH context 3219474
Unmethylated C's in CpG context 2739481
Unmethylated C's in CHG context 4925444
Unmethylated C's in CHH context 19567919
Percentage methylation (CpG context) 26.4%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 14.1%

M-Bias Plot