Alignment Stats
| Sequences analysed in total |
12175584 |
| Single-end alignments with a unique best hit |
5696470 |
| Sequences without alignments under any condition |
199356 |
| Sequences that did not map uniquely |
6279758 |
| Genomic sequence context not extractable (edges of chromosomes) |
3830 |
|
|
Cytosine Methylation
| Total C's analysed |
32257871 |
| Methylated C's in CpG context |
1064240 |
| Methylated C's in CHG context |
167997 |
| Methylated C's in CHH context |
3734153 |
| Methylated C's in Unknown context |
65346 |
| Unmethylated C's in CpG context |
2785236 |
| Unmethylated C's in CHG context |
4981668 |
| Unmethylated C's in CHH context |
19524577 |
| Unmethylated C's in Unknown context |
109623 |
| Percentage methylation (CpG context) |
27.6% |
| Percentage methylation (CHG context) |
3.3% |
| Percentage methylation (CHH context) |
16.1% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
2189758 |
original top strand |
| CTOT |
653682 |
complementary to original top strand |
| CTOB |
654623 |
complementary to original bottom strand |
| OB |
2194577 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
5692640 |
| Unique alignments |
4947534 |
| Duplicates removed |
745106 |
| Duplicated alignments were found at 427088 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
27887572 |
| Methylated C's in CpG context |
904070 |
| Methylated C's in CHG context |
142318 |
| Methylated C's in CHH context |
3079869 |
| Unmethylated C's in CpG context |
2346633 |
| Unmethylated C's in CHG context |
4281157 |
| Unmethylated C's in CHH context |
17133525 |
| Percentage methylation (CpG context) |
27.8% |
| Percentage methylation (CHG context) |
3.2% |
| Percentage methylation (CHH context) |
15.2% |
|
|
M-Bias Plot
