Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/5_ACAGTG_L001_R1_001_trimmed.fq.gz

Data processed at 16:19:34 on 2021-06-03


Alignment Stats

Sequences analysed in total 12231319
Single-end alignments with a unique best hit 5718874
Sequences without alignments under any condition 184967
Sequences that did not map uniquely 6327478
Genomic sequence context not extractable (edges of chromosomes) 3958

Cytosine Methylation

Total C's analysed 32455254
Methylated C's in CpG context 1107493
Methylated C's in CHG context 149103
Methylated C's in CHH context 3559852
Methylated C's in Unknown context 62167
Unmethylated C's in CpG context 2805384
Unmethylated C's in CHG context 5099491
Unmethylated C's in CHH context 19733931
Unmethylated C's in Unknown context 107662
Percentage methylation (CpG context) 28.3%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 15.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2232041 original top strand
CTOT 625277 complementary to original top strand
CTOB 627146 complementary to original bottom strand
OB 2230452 original bottom strand

Deduplication

Alignments analysed 5714916
Unique alignments 4934475
Duplicates removed 780441
Duplicated alignments were found at 442111 different positions

Cytosine Methylation after Extraction

Total C's analysed 27854315
Methylated C's in CpG context 935861
Methylated C's in CHG context 124388
Methylated C's in CHH context 2873426
Unmethylated C's in CpG context 2346339
Unmethylated C's in CHG context 4353881
Unmethylated C's in CHH context 17220420
Percentage methylation (CpG context) 28.5%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 14.3%

M-Bias Plot