Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/4_TGACCA_L001_R1_001_trimmed.fq.gz

Data processed at 16:19:34 on 2021-06-03


Alignment Stats

Sequences analysed in total 11408856
Single-end alignments with a unique best hit 5364918
Sequences without alignments under any condition 163003
Sequences that did not map uniquely 5880935
Genomic sequence context not extractable (edges of chromosomes) 3728

Cytosine Methylation

Total C's analysed 30242630
Methylated C's in CpG context 1073001
Methylated C's in CHG context 126723
Methylated C's in CHH context 3190332
Methylated C's in Unknown context 55634
Unmethylated C's in CpG context 2530272
Unmethylated C's in CHG context 4740036
Unmethylated C's in CHH context 18582266
Unmethylated C's in Unknown context 99087
Percentage methylation (CpG context) 29.8%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 14.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2109625 original top strand
CTOT 570642 complementary to original top strand
CTOB 571132 complementary to original bottom strand
OB 2109791 original bottom strand

Deduplication

Alignments analysed 5361190
Unique alignments 4708897
Duplicates removed 652293
Duplicated alignments were found at 404094 different positions

Cytosine Methylation after Extraction

Total C's analysed 26471633
Methylated C's in CpG context 929005
Methylated C's in CHG context 107697
Methylated C's in CHH context 2661463
Unmethylated C's in CpG context 2180754
Unmethylated C's in CHG context 4130778
Unmethylated C's in CHH context 16461936
Percentage methylation (CpG context) 29.9%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 13.9%

M-Bias Plot