Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11654467 Number of alignments with a unique best hit from the different alignments: 5433833 Mapping efficiency: 46.6% Sequences with no alignments under any condition: 191064 Sequences did not map uniquely: 6029570 Sequences which were discarded because genomic sequence could not be extracted: 3794 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2119792 ((converted) top strand) CT/GA: 2115564 ((converted) bottom strand) GA/CT: 596380 (complementary to (converted) top strand) GA/GA: 598303 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 30793506 Total methylated C's in CpG context: 1054675 Total methylated C's in CHG context: 176932 Total methylated C's in CHH context: 3415378 Total methylated C's in Unknown context: 57018 Total unmethylated C's in CpG context: 2614904 Total unmethylated C's in CHG context: 4783710 Total unmethylated C's in CHH context: 18747907 Total unmethylated C's in Unknown context: 99509 C methylated in CpG context: 28.7% C methylated in CHG context: 3.6% C methylated in CHH context: 15.4% C methylated in Unknown context (CN or CHN): 36.4% Bismark completed in 0d 0h 23m 19s