Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/1_ATCACG_L001_R1_001_trimmed.fq.gz

Data processed at 16:19:34 on 2021-06-03


Alignment Stats

Sequences analysed in total 9246318
Single-end alignments with a unique best hit 4354977
Sequences without alignments under any condition 137109
Sequences that did not map uniquely 4754232
Genomic sequence context not extractable (edges of chromosomes) 3055

Cytosine Methylation

Total C's analysed 24632437
Methylated C's in CpG context 867950
Methylated C's in CHG context 113827
Methylated C's in CHH context 2657818
Methylated C's in Unknown context 46268
Unmethylated C's in CpG context 2076267
Unmethylated C's in CHG context 3866565
Unmethylated C's in CHH context 15050010
Unmethylated C's in Unknown context 80746
Percentage methylation (CpG context) 29.5%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 15.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1705631 original top strand
CTOT 469284 complementary to original top strand
CTOB 472270 complementary to original bottom strand
OB 1704737 original bottom strand

Deduplication

Alignments analysed 4351922
Unique alignments 3838635
Duplicates removed 513287
Duplicated alignments were found at 309988 different positions

Cytosine Methylation after Extraction

Total C's analysed 21669035
Methylated C's in CpG context 747767
Methylated C's in CHG context 92651
Methylated C's in CHH context 2215781
Unmethylated C's in CpG context 1808397
Unmethylated C's in CHG context 3389071
Unmethylated C's in CHH context 13415368
Percentage methylation (CpG context) 29.3%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 14.2%

M-Bias Plot