Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth5_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth5_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 639003 Mapping efficiency: 63.9% Sequence pairs with no alignments under any condition: 241892 Sequence pairs did not map uniquely: 119105 Sequence pairs which were discarded because genomic sequence could not be extracted: 4356 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 150506 ((converted) top strand) GA/CT/CT: 166364 (complementary to (converted) top strand) GA/CT/GA: 167167 (complementary to (converted) bottom strand) CT/GA/GA: 150610 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 29305824 Total methylated C's in CpG context: 363041 Total methylated C's in CHG context: 44799 Total methylated C's in CHH context: 148913 Total methylated C's in Unknown context: 3893 Total unmethylated C's in CpG context: 6096770 Total unmethylated C's in CHG context: 5757922 Total unmethylated C's in CHH context: 16894379 Total unmethylated C's in Unknown context: 54649 C methylated in CpG context: 5.6% C methylated in CHG context: 0.8% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 6.6% Bismark completed in 0d 0h 9m 37s