Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth4_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth4_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 1000000 Number of paired-end alignments with a unique best hit: 607658 Mapping efficiency: 60.8% Sequence pairs with no alignments under any condition: 278310 Sequence pairs did not map uniquely: 114032 Sequence pairs which were discarded because genomic sequence could not be extracted: 5900 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 144454 ((converted) top strand) GA/CT/CT: 156290 (complementary to (converted) top strand) GA/CT/GA: 156371 (complementary to (converted) bottom strand) CT/GA/GA: 144643 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 27381443 Total methylated C's in CpG context: 358464 Total methylated C's in CHG context: 55587 Total methylated C's in CHH context: 189206 Total methylated C's in Unknown context: 3538 Total unmethylated C's in CpG context: 5701514 Total unmethylated C's in CHG context: 5372099 Total unmethylated C's in CHH context: 15704573 Total unmethylated C's in Unknown context: 51411 C methylated in CpG context: 5.9% C methylated in CHG context: 1.0% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 6.4% Bismark completed in 0d 0h 9m 21s