Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth9_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth9_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 87033711 Number of paired-end alignments with a unique best hit: 42616137 Mapping efficiency: 49.0% Sequence pairs with no alignments under any condition: 39456215 Sequence pairs did not map uniquely: 4961359 Sequence pairs which were discarded because genomic sequence could not be extracted: 161186 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10341093 ((converted) top strand) GA/CT/CT: 10826509 (complementary to (converted) top strand) GA/CT/GA: 10905180 (complementary to (converted) bottom strand) CT/GA/GA: 10382169 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1829339257 Total methylated C's in CpG context: 122087556 Total methylated C's in CHG context: 6932645 Total methylated C's in CHH context: 22774872 Total methylated C's in Unknown context: 1338648 Total unmethylated C's in CpG context: 140186851 Total unmethylated C's in CHG context: 342382683 Total unmethylated C's in CHH context: 1194974650 Total unmethylated C's in Unknown context: 6684213 C methylated in CpG context: 46.5% C methylated in CHG context: 2.0% C methylated in CHH context: 1.9% C methylated in unknown context (CN or CHN): 16.7% Bismark completed in 0d 7h 8m 19s