Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth5_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth5_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 284116370 Number of paired-end alignments with a unique best hit: 181427646 Mapping efficiency: 63.9% Sequence pairs with no alignments under any condition: 68552902 Sequence pairs did not map uniquely: 34135822 Sequence pairs which were discarded because genomic sequence could not be extracted: 1236735 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 42968517 ((converted) top strand) GA/CT/CT: 47095015 (complementary to (converted) top strand) GA/CT/GA: 47244227 (complementary to (converted) bottom strand) CT/GA/GA: 42883152 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 8278073563 Total methylated C's in CpG context: 103088093 Total methylated C's in CHG context: 12751693 Total methylated C's in CHH context: 42521811 Total methylated C's in Unknown context: 1099911 Total unmethylated C's in CpG context: 1724103076 Total unmethylated C's in CHG context: 1627995440 Total unmethylated C's in CHH context: 4767613450 Total unmethylated C's in Unknown context: 15496450 C methylated in CpG context: 5.6% C methylated in CHG context: 0.8% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 6.6% Bismark completed in 1d 4h 42m 0s