Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth8_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth8_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 50248 Mapping efficiency: 50.2% Sequence pairs with no alignments under any condition: 43383 Sequence pairs did not map uniquely: 6369 Sequence pairs which were discarded because genomic sequence could not be extracted: 147 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12176 ((converted) top strand) GA/CT/CT: 12652 (complementary to (converted) top strand) GA/CT/GA: 13038 (complementary to (converted) bottom strand) CT/GA/GA: 12235 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2362246 Total methylated C's in CpG context: 289097 Total methylated C's in CHG context: 11578 Total methylated C's in CHH context: 34199 Total methylated C's in Unknown context: 1726 Total unmethylated C's in CpG context: 74819 Total unmethylated C's in CHG context: 455380 Total unmethylated C's in CHH context: 1497173 Total unmethylated C's in Unknown context: 7374 C methylated in CpG context: 79.4% C methylated in CHG context: 2.5% C methylated in CHH context: 2.2% C methylated in unknown context (CN or CHN): 19.0% Bismark completed in 0d 0h 4m 11s