Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth4_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth4_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 60630 Mapping efficiency: 60.6% Sequence pairs with no alignments under any condition: 27923 Sequence pairs did not map uniquely: 11447 Sequence pairs which were discarded because genomic sequence could not be extracted: 603 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14325 ((converted) top strand) GA/CT/CT: 15556 (complementary to (converted) top strand) GA/CT/GA: 15685 (complementary to (converted) bottom strand) CT/GA/GA: 14461 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2740471 Total methylated C's in CpG context: 35671 Total methylated C's in CHG context: 5551 Total methylated C's in CHH context: 19320 Total methylated C's in Unknown context: 374 Total unmethylated C's in CpG context: 569701 Total unmethylated C's in CHG context: 539060 Total unmethylated C's in CHH context: 1571168 Total unmethylated C's in Unknown context: 5073 C methylated in CpG context: 5.9% C methylated in CHG context: 1.0% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 6.9% Bismark completed in 0d 0h 4m 15s