Bismark report for: /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth1_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/041720-pan-u/Pact_trim/Meth1_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pan_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 49439 Mapping efficiency: 49.4% Sequence pairs with no alignments under any condition: 46001 Sequence pairs did not map uniquely: 4560 Sequence pairs which were discarded because genomic sequence could not be extracted: 213 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11449 ((converted) top strand) GA/CT/CT: 12930 (complementary to (converted) top strand) GA/CT/GA: 13090 (complementary to (converted) bottom strand) CT/GA/GA: 11757 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2105037 Total methylated C's in CpG context: 15600 Total methylated C's in CHG context: 4256 Total methylated C's in CHH context: 16299 Total methylated C's in Unknown context: 880 Total unmethylated C's in CpG context: 305053 Total unmethylated C's in CHG context: 373438 Total unmethylated C's in CHH context: 1390391 Total unmethylated C's in Unknown context: 7283 C methylated in CpG context: 4.9% C methylated in CHG context: 1.1% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 10.8% Bismark completed in 0d 0h 4m 10s