Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM05-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM05-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 48548054 Number of paired-end alignments with a unique best hit: 4410419 Mapping efficiency: 9.1% Sequence pairs with no alignments under any condition: 40594655 Sequence pairs did not map uniquely: 3542980 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1139379 ((converted) top strand) GA/CT/CT: 1074108 (complementary to (converted) top strand) GA/CT/GA: 1066348 (complementary to (converted) bottom strand) CT/GA/GA: 1130577 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 76674394 Total methylated C's in CpG context: 1156945 Total methylated C's in CHG context: 231352 Total methylated C's in CHH context: 4537735 Total methylated C's in Unknown context: 138959 Total unmethylated C's in CpG context: 7723284 Total unmethylated C's in CHG context: 12186707 Total unmethylated C's in CHH context: 50838371 Total unmethylated C's in Unknown context: 731315 C methylated in CpG context: 13.0% C methylated in CHG context: 1.9% C methylated in CHH context: 8.2% C methylated in unknown context (CN or CHN): 16.0% Bismark completed in 0d 8h 16m 19s