Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM01-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF05-EM01-Larvae_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 39294742 Number of paired-end alignments with a unique best hit: 11922548 Mapping efficiency: 30.3% Sequence pairs with no alignments under any condition: 21460435 Sequence pairs did not map uniquely: 5911759 Sequence pairs which were discarded because genomic sequence could not be extracted: 3 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2903527 ((converted) top strand) GA/CT/CT: 3054689 (complementary to (converted) top strand) GA/CT/GA: 3059526 (complementary to (converted) bottom strand) CT/GA/GA: 2904803 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 399118445 Total methylated C's in CpG context: 11209649 Total methylated C's in CHG context: 1135547 Total methylated C's in CHH context: 6939128 Total methylated C's in Unknown context: 307446 Total unmethylated C's in CpG context: 48189502 Total unmethylated C's in CHG context: 82581213 Total unmethylated C's in CHH context: 249063406 Total unmethylated C's in Unknown context: 1774410 C methylated in CpG context: 18.9% C methylated in CHG context: 1.4% C methylated in CHH context: 2.7% C methylated in unknown context (CN or CHN): 14.8% Bismark completed in 0d 14h 38m 45s