Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF07-CM02-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF07-CM02-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 60588756 Number of paired-end alignments with a unique best hit: 7587682 Mapping efficiency: 12.5% Sequence pairs with no alignments under any condition: 48271147 Sequence pairs did not map uniquely: 4729927 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2112107 ((converted) top strand) GA/CT/CT: 1728030 (complementary to (converted) top strand) GA/CT/GA: 1685694 (complementary to (converted) bottom strand) CT/GA/GA: 2061844 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 176275607 Total methylated C's in CpG context: 3938080 Total methylated C's in CHG context: 607095 Total methylated C's in CHH context: 9685016 Total methylated C's in Unknown context: 289267 Total unmethylated C's in CpG context: 17009743 Total unmethylated C's in CHG context: 30870019 Total unmethylated C's in CHH context: 114165654 Total unmethylated C's in Unknown context: 961142 C methylated in CpG context: 18.8% C methylated in CHG context: 1.9% C methylated in CHH context: 7.8% C methylated in unknown context (CN or CHN): 23.1% Bismark completed in 0d 12h 25m 45s