Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM02-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF05-CM02-Larvae_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53936905 Number of paired-end alignments with a unique best hit: 19268727 Mapping efficiency: 35.7% Sequence pairs with no alignments under any condition: 24852305 Sequence pairs did not map uniquely: 9815873 Sequence pairs which were discarded because genomic sequence could not be extracted: 14 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4540889 ((converted) top strand) GA/CT/CT: 5088529 (complementary to (converted) top strand) GA/CT/GA: 5090569 (complementary to (converted) bottom strand) CT/GA/GA: 4548726 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 632232949 Total methylated C's in CpG context: 17125121 Total methylated C's in CHG context: 1631911 Total methylated C's in CHH context: 9284949 Total methylated C's in Unknown context: 307839 Total unmethylated C's in CpG context: 76864129 Total unmethylated C's in CHG context: 130104233 Total unmethylated C's in CHH context: 397222606 Total unmethylated C's in Unknown context: 2612278 C methylated in CpG context: 18.2% C methylated in CHG context: 1.2% C methylated in CHH context: 2.3% C methylated in unknown context (CN or CHN): 10.5% Bismark completed in 0d 22h 17m 26s