Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM05-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM05-Larvae_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 48978052 Number of paired-end alignments with a unique best hit: 17211018 Mapping efficiency: 35.1% Sequence pairs with no alignments under any condition: 23481673 Sequence pairs did not map uniquely: 8285361 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4221351 ((converted) top strand) GA/CT/CT: 4375606 (complementary to (converted) top strand) GA/CT/GA: 4385737 (complementary to (converted) bottom strand) CT/GA/GA: 4228322 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 576011240 Total methylated C's in CpG context: 17042772 Total methylated C's in CHG context: 3377754 Total methylated C's in CHH context: 14831225 Total methylated C's in Unknown context: 430560 Total unmethylated C's in CpG context: 69075608 Total unmethylated C's in CHG context: 118450863 Total unmethylated C's in CHH context: 353233018 Total unmethylated C's in Unknown context: 2500352 C methylated in CpG context: 19.8% C methylated in CHG context: 2.8% C methylated in CHH context: 4.0% C methylated in unknown context (CN or CHN): 14.7% Bismark completed in 0d 20h 25m 34s