Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM03-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF03-CM03-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 64470157 Number of paired-end alignments with a unique best hit: 16517068 Mapping efficiency: 25.6% Sequence pairs with no alignments under any condition: 38359026 Sequence pairs did not map uniquely: 9594063 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4608378 ((converted) top strand) GA/CT/CT: 3664120 (complementary to (converted) top strand) GA/CT/GA: 3651795 (complementary to (converted) bottom strand) CT/GA/GA: 4592768 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 372908976 Total methylated C's in CpG context: 9796068 Total methylated C's in CHG context: 1721896 Total methylated C's in CHH context: 15173231 Total methylated C's in Unknown context: 374581 Total unmethylated C's in CpG context: 36640081 Total unmethylated C's in CHG context: 67610053 Total unmethylated C's in CHH context: 241967647 Total unmethylated C's in Unknown context: 1970981 C methylated in CpG context: 21.1% C methylated in CHG context: 2.5% C methylated in CHH context: 5.9% C methylated in unknown context (CN or CHN): 16.0% Bismark completed in 0d 19h 40m 29s