Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF02-CM02-Zygote_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/CF02-CM02-Zygote_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 67063678 Number of paired-end alignments with a unique best hit: 10105983 Mapping efficiency: 15.1% Sequence pairs with no alignments under any condition: 50657462 Sequence pairs did not map uniquely: 6300233 Sequence pairs which were discarded because genomic sequence could not be extracted: 6 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3000851 ((converted) top strand) GA/CT/CT: 2072592 (complementary to (converted) top strand) GA/CT/GA: 2052690 (complementary to (converted) bottom strand) CT/GA/GA: 2979844 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 188887969 Total methylated C's in CpG context: 4107683 Total methylated C's in CHG context: 1286993 Total methylated C's in CHH context: 13086957 Total methylated C's in Unknown context: 266487 Total unmethylated C's in CpG context: 16031720 Total unmethylated C's in CHG context: 29129543 Total unmethylated C's in CHH context: 125245073 Total unmethylated C's in Unknown context: 1213471 C methylated in CpG context: 20.4% C methylated in CHG context: 4.2% C methylated in CHH context: 9.5% C methylated in unknown context (CN or CHN): 18.0% Bismark completed in 0d 14h 42m 49s