Bismark report for: /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM04-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM04-Larvae_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 62743331 Number of paired-end alignments with a unique best hit: 22325576 Mapping efficiency: 35.6% Sequence pairs with no alignments under any condition: 27547702 Sequence pairs did not map uniquely: 12870053 Sequence pairs which were discarded because genomic sequence could not be extracted: 21 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5482249 ((converted) top strand) GA/CT/CT: 5667672 (complementary to (converted) top strand) GA/CT/GA: 5684768 (complementary to (converted) bottom strand) CT/GA/GA: 5490866 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 644792517 Total methylated C's in CpG context: 19814115 Total methylated C's in CHG context: 2991493 Total methylated C's in CHH context: 16246243 Total methylated C's in Unknown context: 475752 Total unmethylated C's in CpG context: 76585909 Total unmethylated C's in CHG context: 133823662 Total unmethylated C's in CHH context: 395331095 Total unmethylated C's in Unknown context: 2728318 C methylated in CpG context: 20.6% C methylated in CHG context: 2.2% C methylated in CHH context: 3.9% C methylated in unknown context (CN or CHN): 14.8% Bismark completed in 0d 3h 36m 50s