Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM04-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM04-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 62743331
Paired-end alignments with a unique best hit 22325576
Pairs without alignments under any condition 27547702
Pairs that did not map uniquely 12870053
Genomic sequence context not extractable (edges of chromosomes) 21

Cytosine Methylation

Total C's analysed 644792517
Methylated C's in CpG context 19814115
Methylated C's in CHG context 2991493
Methylated C's in CHH context 16246243
Methylated C's in Unknown context 475752
Unmethylated C's in CpG context 76585909
Unmethylated C's in CHG context 133823662
Unmethylated C's in CHH context 395331095
Unmethylated C's in Unknown context 2728318
Percentage methylation (CpG context) 20.6%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5482249 original top strand
CTOT 5667672 complementary to original top strand
CTOB 5684768 complementary to original bottom strand
OB 5490866 original bottom strand

Deduplication

Alignments analysed 22325555
Unique alignments 16804375
Duplicates removed 5521180
Duplicated alignments were found at 4120941 different positions

Cytosine Methylation after Extraction

Total C's analysed 262376593
Methylated C's in CpG context 7910157
Methylated C's in CHG context 1257384
Methylated C's in CHH context 6578903
Unmethylated C's in CpG context 31080214
Unmethylated C's in CHG context 54129192
Unmethylated C's in CHH context 161420743
Percentage methylation (CpG context) 20.3%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 3.9%

M-Bias Plot