Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM03-Larvae_R1_001_val_1.fq.gz and /gscratch/scrubbed/samwhite/2018OALarvae_DNAm_discovery/trimmed_files/EF08-EM03-Larvae_R2_001_val_2.fq.gz

Data processed at 00:16 on 2021-05-09


Alignment Stats

Sequence pairs analysed in total 50784782
Paired-end alignments with a unique best hit 18568101
Pairs without alignments under any condition 21834010
Pairs that did not map uniquely 10382671
Genomic sequence context not extractable (edges of chromosomes) 3

Cytosine Methylation

Total C's analysed 584116241
Methylated C's in CpG context 16830309
Methylated C's in CHG context 1338341
Methylated C's in CHH context 8845420
Methylated C's in Unknown context 253358
Unmethylated C's in CpG context 69072886
Unmethylated C's in CHG context 120499030
Unmethylated C's in CHH context 367530255
Unmethylated C's in Unknown context 2169583
Percentage methylation (CpG context) 19.6%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 2.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4363996 original top strand
CTOT 4900240 complementary to original top strand
CTOB 4920484 complementary to original bottom strand
OB 4383378 original bottom strand

Deduplication

Alignments analysed 18568098
Unique alignments 12501093
Duplicates removed 6067005
Duplicated alignments were found at 3930394 different positions

Cytosine Methylation after Extraction

Total C's analysed 226007355
Methylated C's in CpG context 6138664
Methylated C's in CHG context 503168
Methylated C's in CHH context 3098933
Unmethylated C's in CpG context 26636206
Unmethylated C's in CHG context 45933567
Unmethylated C's in CHH context 143696817
Percentage methylation (CpG context) 18.7%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 2.1%

M-Bias Plot